AASLD 2013: Tenofovir Alafenamide Shows Similar Anti-HBV Activity with Less Kidney Toxicity


A new formulation of tenofovir that can be taken at lower doses demonstrated potent activity against hepatitis B virus (HBV) similar to that of the existing formulation in a 28-day study, but with less effect on kidney function, researchers reported at the 64thAASLD Liver Meeting this month in Washington, DC.

Gilead Science's tenofovir disoproxil fumarate (TDF, brand name Viread) -- the currently marketed formulation -- is one of the most effective antiviral drugs for hepatitis B as well as one of the most widely used antiretrovirals for HIV. TDF is generally safe and well-tolerated, but it can cause kidney toxicity in susceptible individuals and is also associated with bone loss.

A new pro-drug formulation known as tenofovir alafenamide (TAF, formerly GS-7340) produces 5-fold higher concentrations of active tenofovir diphosphate in lymphoid cells that harbor HIV, but is more stable in plasma and results in lower blood drug levels and systemic exposures than those seen with TDF. This allows for reduced dosing with potentially less detrimental effect on the kidneys and bones.

Over the past year researchers have reported that a 10 mg dose of TAF is as effective against HIV as a 300 mg dose of TDF through 48 weeks in Phase 2 studies, but the former has less impact on markers of kidney function and bone mineral density.

TAF also leads to higher levels of active tenofovir in hepatocytes (liver cells). At the Liver Meeting, Josh Agarwal from Kings College Hospital in London and colleagues reported findings from a study comparing TAF versus TDF for the treatment of chronic HBV infection.

This open-label Phase 1b study enrolled 51 chronic hepatitis B patients with HBV DNA levels of at least 2000 IU/mL. Most participants were men, about 60% were Asian, about 30% were black, and approximately half were hepatitis B "e" antigen (HBeAg) negative. At baseline they had ALT levels within 10 x the upper limit of normal and creatine clearance >70 mL/min.

Participants were randomly allocated to equal-sized arms receiving TAF at doses of 8, 25, 40, or 120 mg, or TDF at a dose of 300 mg, for 28 days, with 4 weeks of post-treatment follow-up.


"Over a 28-day dosing period, TAF was safe and well-tolerated in patients with chronic HBV," the researchers concluded. "The antiviral activity of TAF did not vary by dose and was comparable to TDF."

Further clinical trials of TAF for chronic hepatitis B are planned using a selected dose of 25 mg.



K Agarwal, SK Fung, TT Nguyen, et al.Twenty-Eight Day Safety and Efficacy of Tenofovir Alafenamide (TAF) Fumarate in Chronic Hepatitis B (CHB) Patients. 64th Annual Meeting of the American Association for the Study of Liver Diseases (AASLD 2013). Washington, DC, November 1-5, 2013. Abstract973.