Back HCV Treatment AASLD 2012: Sofosbuvir + GS-5885 + Ribavirin Shows High Early Response Rates for Genotype 1 HCV

AASLD 2012: Sofosbuvir + GS-5885 + Ribavirin Shows High Early Response Rates for Genotype 1 HCV

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The hepatitis C virus polymerase inhibitor sofosbuvir produces high rates of sustained virological response for genotype 1 patients when combined with ribavirin and the HCV NS5A inhibitor GS-5885, according to data from the ELECTRON trial presented at the American Association for the Study of Liver Diseases Liver Meeting (AASLD 2012) this week in Boston.

The recent approval of the first direct-acting hepatitis C drugs has ushered in a new era of treatment, but many patients and providers eagerly await all-oral therapy that avoids interferon and its difficult side effects. Several combinations of new direct-acting agents are under study, but response rates remain suboptimal for the most difficult-to-treat patients.

Sofosbuvir/Ribavirin So Far

Gilead Science's sofosbuvir -- formerly known as GS-7977, and before that as PSI-7977 prior to Gilead's acquisition of Pharmasset -- is a once-daily uridine nucleotide analog that blocks the activity of the HCV NS5B polymerase, which copies viral genetic material. Early studies showed that it has potent activity against hepatitis C, has a high barrier to resistance, and is generally safe and well-tolerated.

Edward Gane from Auckland City Hospital in New Zealand described the latest data from the Phase 2 ELECTRON trial, which is evaluating sofosbuvir in various combinations.

As background, Gane noted that researchers initially looked at whether sofosbuvir works without pegylated interferon, starting with the easiest-to-treat patients: previously untreated people with HCV genotypes 2 or 3.

As Gane reported at last year's AASLD meeting, a dual oral regimen of 400 mg sofosbuvir plus 1000-1200 mg weight-based ribavirin for 12 weeks led to 100% sustained virological response 24 weeks after finishing treatment (SVR24), the traditional definition of a cure.

However, sofosbuvir monotherapy or sofosbuvir plus reduced-dose (800 mg) ribavirin for 12 weeks, or sofosbuvir plus standard-dose ribavirin for 8 weeks, only cured about 60% of treatment-naive genotype 2/3 patients. The SVR24 rate was not much higher -- 68% -- for treatment-experienced genotype 2/3 patients (prior relapsers, partial responders, and null responders combined) treated with the 12-week, standard-dose ribavirin regimen.

HCV Genotype 1

The ELECTRON team then proceeded to test sofosbuvir in people with more difficult-to-treat HCV genotype 1, both treatment-naive and null responders to previous interferon-based therapy. They looked first at the same combination of 400 mg sofosbuvir once-daily plus 1000-1200 mg ribavirin for 12 weeks.

As Gane reported at this year's Retrovirus conference, although all participants saw a rapid decline in HCV RNA and had undetectable viral load at the end of the 12-week course of treatment, almost all genotype 1 prior null responders relapsed soon thereafter, resulting in a very low cure rate.

Given that sofosbuvir plus ribavirin as a dual regimen is not adequate for this more difficult-to-treat patient population, researchers then asked whether adding a second direct-acting agent could increase cure rates.

An additional 2 ELECTRON arms were added to test a 12-week triple regimen of sofosbuvir plus ribavirin plus GS-5885, Gilead's HCV NS5A replication complex inhibitor, in treatment-naive patients and prior null responders. In studies to date GS-5885 has shown good activity against hepatitis C -- including virus with the signature S282T polymerase resistance mutation -- as well as a favorable safety and tolerability profile.

At this week's meeting Gane presented data from the 4 ELECTRON genotype 1 arms evaluated to date:

  • Sofosbuvir + ribavirin for 12 weeks in 25 treatment-naive patients;
  • Sofosbuvir + ribavirin for 12 weeks in 10 prior null responders;
  • Sofosbuvir + GS-5885 + ribavirin for 12 weeks in 25 treatment-naive patients;
  • Sofosbuvir + GS-5885 + ribavirin for 12 weeks in 9 prior null responders.

About 65% of study participants in the sofosbuvir/ribavirin arms were men; in the triple-therapy arms, only 32% of treatment-naive patients were men, rising to 78% of prior null responders. Across all arms, most participants (80%-100%) were white and the average age was similar at approximately 48 years.

Nearly 90% of participants had difficult-to-treat HCV subtype 1a -- typical of the hepatitis C population in Australia, Gane noted. None had liver cirrhosis. Among treatment-naive patients assigned to either regimen, about 40% had the IL28B CC gene variant associated with good response to interferon; as expected, only 20% of null responders in the dual-therapy arm and none in the triple-therapy arm had this favorable pattern.

Results

  • As seen in the earlier study arms, almost all participants experienced rapid virological response (RVR), or undetectable HCV viral load by week 4 of treatment.
  • All patients -- including the single individual who did not have RVR at week 4 -- had HCV RNA below 15 IU/mL at week 12, for an end-of-treatment response rate of 100% across all arms.
  • No participants experienced viral breakthrough during treatment.
  • Outcomes diverged dramatically, however, after completing treatment.
  • Among recipients of sofosbuvir/ribavirin dual therapy, 4 treatment-naive people and 9 out of 10 prior null responders experienced viral relapse within 4 weeks post-treatment, yielding SVR4 rates of 88% and 10%, respectively.
  • An additional treatment-naive patient relapsed later, resulting in 12-week sustained virological response (SVR12) rates of 84% and 10%, respectively.
  • In contrast, the 3-drug regimen containing GS-5885 continued to perform well.
  • None of the 25 total participants in the treatment-naive arm, nor the 3 patients who reached this time point in the null responder arm, relapsed by post-treatment week 4, so SVR4 rates remained at 100%.
  • Both regimens were generally safe and well-tolerated.
  • Side effects were uncommon, and they mostly occurred with similar frequency across study arms.
  • Anemia was more common (20%) in the treatment-naive triple therapy arm -- the only one with high proportion of women --and about one-third of people in this arm had unexplained blood in their urine; however, none of the null responders taking the same regimen experienced either symptom.
  • There was 1 serious adverse event in the dual-therapy arm and 2 in the triple-therapy arm -- none judged by investigators to be drug-related -- and only 1 person in the latter group discontinued treatment early for this reason.
  • No participants had severe grade 4 laboratory abnormalities.

"In HCV genotype 2 or 3 infection, sofosbuvir + ribavirin for 12 weeks appears to be a safe and effective regimen for both treatment-naive and previously treated patients," the researchers summarized. "In HCV genotype 1 infection, sofosbuvir + GS-5885 + ribavirin for 12 weeks appears to be a safe and effective regimen for treatment-naive patients."

Overall, Gane said, addition of GS-5885 to sofosbuvir/ribavirin introduced "no additional safety issues."

Week 4 after the end of therapy is too soon to determine whether participants are in fact cured, but these results are promising given that most relapses in the dual-therapy arm occurred by this point.

Based on these findings, adding GS-5885 appears to prevent post-treatment relapse among people who fully suppress HCV during treatment. Ribavirin has a similar effect, though it was not adequate for genotype 1 patients in this study.

It will be interesting to see whether a dual regimen of sofosbuvir plus GS-5885 without ribavirin will produce similar results in difficult-to-treat patients, as the elimination of ribavirin could reduce side effects, especially anemia.

Another study presented at the AASLD meeting showed that a dual regimen of sofosbuvir plus a different NS5A inhibitor, Bristol-Myers Squibb's daclatasvir, also demonstrated high sustained response rates, and the addition of ribavirin did not add to its efficacy.

Gilead indicated this past spring that it would no longer pursue studies of the sofosbuvir/daclatasvir combination, leading to speculation that it planned to focus on its own NS5A inhibitor candidates which could be coformulated into fixed-dose tablets -- the company's specialty in the HIV arena.

In a press release issued to coincide with this week's meeting, Gilead said it recently initiated a Phase 3 trial -- dubbed ION-I -- to evaluate a fixed-dose combination of sofosbuvir plus GS-5885, with and without ribavirin, for 12 and 24 weeks duration, in treatment-naive genotype 1 chronic hepatitis C patients.

11/15/12

Reference

EJ Gane, CA Stedman, RH Hyland, et al. Once Daily Sofosbuvir (GS-7977) Plus Ribavirin in Patients with HCV Genotypes 1, 2, and 3: The ELECTRON Trial. 63rd Annual Meeting of the American Association for the Study of Liver Diseases (AASLD 2012). Boston, November 9-13, 2012. Abstract 229.

Other Source

Gilead Sciences. Gilead Announces 100 Percent Sustained Virologic Response Rate (SVR4) for an Interferon-Free Regimen of Sofosbuvir (GS-7977), GS-5885 and Ribavirin in Treatment-Naive Genotype 1 Hepatitis C Infected Patients. Press release. November 10, 2012.