- Category: HIV Basic Science
- Published on Tuesday, 06 May 2014 00:00
- Written by ASBMB
A set of proteins derived from soft coral identified from a National Cancer Institute repository of biological chemicals can bind to HIV and prevent it entering immune cells, suggesting it may have potential for use in microbicide gels or other products to prevent transmission of the virus, according to a poster presented at the Experimental Biology 2014 meeting last week in San Diego.
NCI fellow Koreen Ramessarand colleagues identified 3 proteins -- CNID-1, CNID-2 and CNID-3 -- that inhibit viral fusion with CD4 T-cells, with the antiviral effect occurring after initial virus-to-cell attachment but prior to viral entry.
"Cnidarins represent a novel class of proteins that target viral fusion and inhibit HIV infection in a manner different from any currently identified anti-HIV agent," the researchers concluded.
Below is an edited excerpt from a press release issued by the American Society for Biochemistry and Molecular Biology describing the research in more detail.
Coral Reefs Provide Potent New Anti-HIV Proteins
Discovery raises hope for new methods to prevent the spread of HIV
San Diego -- April 29, 2014 -- Researchers have discovered a new class of proteins capable of blocking the HIV virus from penetrating T-cells, raising hope that the proteins could be adapted for use in gels or sexual lubricants to provide a potent barrier against HIV infection.
The proteins, called cnidarins, were found in a feathery coral collected in waters off Australia's northern coast. Researchers zeroed in on the proteins after screening thousands of natural product extracts in a biorepository maintained by the National Cancer Institute.
"It's always thrilling when you find a brand-new protein that nobody else has ever seen before," said senior investigator Barry O'Keefe, PhD, deputy chief of the Molecular Targets Laboratory at the National Cancer Institute's Center for Cancer Research. "And the fact that this protein appears to block HIV infection -- and to do it in a completely new way -- makes this truly exciting."
In the global fight against AIDS, there is a pressing need for anti-HIV microbicides that women can apply to block HIV infection without relying on a man's willingness to use a condom. Koreen Ramessar, PhD, a postdoctoral research fellow at the National Cancer Institute and a member of the research team, said cnidarins could be ideally suited for use in such a product because the proteins block HIV transmission without encouraging the virus to become resistant to other HIV drugs.
"When developing new drugs, we're always concerned about the possibility of undermining existing successful treatments by encouraging drug resistance in the virus," said O'Keefe. "But even if the virus became resistant to these proteins, it would likely still be sensitive to all of the therapeutic options that are currently available."
The research team identified and purified the cnidarin proteins, then tested their activity against laboratory strains of HIV. The proteins proved astonishingly potent, capable of blocking HIV at concentrations of a billionth of a gram by preventing the first step in HIV transmission, in which the virus must enter a type of immune cell known as the T-cell.
"We found that cnidarins bind to the virus and prevent it from fusing with the T-cell membrane," said Ramessar. "This is completely different from what we've seen with other proteins, so we think the cnidarin proteins have a unique mechanism of action."
The next step is to refine methods for generating cnidarins in larger quantities so the proteins can be tested further to identify potential side effects or activity against other viruses. "Making more of it is a big key," said O'Keefe. "You can't strip the Earth of this coral trying to harvest this protein, so our focus now is on finding ways to produce more of it so we can proceed with preclinical testing."
The scientists discovered cnidarins while screening for proteins, a largely understudied component of natural product extracts found in the National Cancer Institute's extract repository. The institute maintains a large collection of natural specimens gathered from around the world under agreements with their countries of origin. The specimens are available to researchers across the United States.
"The natural products extract repository is a national treasure," said O'Keefe. "You never know what you might find. Hopefully, discoveries like this will encourage more investigators to use this resource to identify extracts with activity against infectious disease."
About Experimental Biology 2014
Experimental Biology is an annual meeting comprised of more than 14,000 scientists and exhibitors from six sponsoring societies and multiple guest societies. With a mission to share the newest scientific concepts and research findings shaping clinical advances, the meeting offers an unparalleled opportunity for exchange among scientists from across the United States and the world who represent dozens of scientific areas, from laboratory to translational to clinical research. http://www.experimentalbiology.org
About the American Society for Biochemistry and Molecular Biology (ASBMB)
ASBMB is a nonprofit scientific and educational organization with more than 12,000 members worldwide. Founded in 1906 to advance the science of biochemistry and molecular biology, the society publishes three peer-reviewed journals, advocates for funding of basic research and education, supports science education at all levels, and promotes the diversity of individuals entering the scientific workforce. http://www.asbmb.org
K Ramessar, CY Xiong, LH Krumpe, BR O'Keefe, et al. Isolation and characterization of a novel class of potent anti-HIV proteins from an Australian soft coral. Experimental Biology 2014. San Diego, April 16-30, 2014. Abstract D208.
American Society for Biochemistry and Molecular Biology. Coral Reefs Provide Potent New Anti-HIV Proteins. Press release. April 29, 2104.