Back HIV Prevention Pre-exposure (PrEP) CROI 2015: Maraviroc Levels in Vaginal and Rectal Tissues May Not Be High Enough for PrEP

CROI 2015: Maraviroc Levels in Vaginal and Rectal Tissues May Not Be High Enough for PrEP

alt

Levels of the HIV entry inhibitor maraviroc (Selzentry) in vaginal and rectal tissues did not reach high enough levels with a single oral dose to confer protection against HIV in a laboratory study, researchers reported at the 2015 Conference on Retroviruses and Opportunistic Infections (CROI) last week in Seattle. Multiple doses, however, could still potentially be effective for pre-exposure prophylaxis, or PrEP.

Pre-exposure prophylaxis using antiretroviral agents offers a way for HIV negative people to protect themselves from HIV infection. Currently oral tenofovir/emtricitabine (Truvada) is the only antiretroviral regimen that has been extensively tested in humans and approved for this purpose, but researchers are exploring other agents in various forms (e.g., pills, gels, vaginal rings).

Maraviroc could be an attractive option for HIV prevention because it acts at an early stage, blocking HIV from attaching to the CCR5 coreceptor that most HIV uses to enter cells; a drawback is that it might not protect against the minority of HIV strains that use the alternate CXCR4 coreceptor. Tenofovir and emtricitabine, in contrast, interfere with viral replication after HIV has already entered a host cell. Maraviroc is known to be generally safe and well-tolerated without the risk of kidney and bone toxicity associated with tenofovir. The safety of oral maraviroc for prevention in HIV negative people is currently being studied in the HPTN 069 Next-PrEP trial.

Julie Fox of Guys and St. Thomas NHS Foundation Trust presented findings froma Phase 4 pharmacokinetic/pharmacodynamic (PK/PD) study to test maraviroc levels in vaginal and rectal tissue after 1 dose and whether it protects against HIV infection. Adequate levels after a single could be important for event-driven or "on-demand" PrEP. Some animal and human studies suggest that drug accumulation in vaginal or rectal tissue plays a role in PrEP efficacy, though studies of oral tenofovir/emtricitabine have mostly correlated effectiveness with drug levels in the blood.

Prior maraviroc prevention research has produced inconsistent findings. Some studies have shown that maraviroc is rapidly absorbed in the genital/anal tract and prevents HIV infection of tissue explants, or tissue samples obtained from humans and maintained in the laboratory. However, a macaque monkey study found that maraviroc failed to protect against rectal exposure to an HIV-like virus.

This open-label study enrolled 54 healthy HIV negative adults (29 men and 25 women) with no sexually transmitted infections at pre-entry screening. The median age was 32 years, two-thirds were white, and 28% were black.

Participants in 4 intervention arms took a single 300 mg oral dose of maraviroc while a control group received a placebo pill. They were randomly assigned to undergo fluid and tissue sampling at 2, 4, 6, or 12 hours after dosing. After a 4-week "washout" period they received another 300 mg dose of maraviroc and had tissue samples collected after 24, 36, 48, or 72 hours.

The researchers measured maraviroc concentrations in participants' blood, rectal fluid, urethral fluid from a swab, cervical-vaginal fluid, and vaginal and rectal tissue biopsy samples. Fluid and tissue samples were either frozen immediately or placed in a preservative solution (phosphate-buffered saline) and transported on ice to the Imperial College pharmacodynamics laboratory, with a median transport time of 30 minutes.

Vaginal and rectal tissue biopsy cuts were then cultured in the lab and exposed to CCR5-tropic HIV-1 (virus using the CCR5 co-receptor) and incubated for 2 hours. Over 15 days of culturing the investigators measured p24 antigen levels, an early marker of HIV replication.

Results

  • Maraviroc was safe and well-tolerated, with no reported side effects.
  • Maraviroc reached maximum concentrations in blood plasma, vaginal tissue, and rectal tissue at 4 hours after dosing.
  • Vaginal tissue concentrations were 3.6 times higher than plasma levels at 4 hours, and 26.0 times higher at 72 hours.
  • Rectal tissue concentrations were 9.7 times higher than plasma at 4 hours and 19.0 times higher at 12 hours, but equalized by 72 hours.
  • Plasma and vaginal maraviroc levels were more closely correlated than plasma and rectal levels (r2 = 0.66 and 0.39, respectively).
  • When vaginal tissue samples were exposed to HIV, p24 levels were reduced at 2 hours post-dosing, indicating some antiviral activity, but this effect was lost by 4 hours.
  • In exposed rectal tissue p24 levels remained high at all time points, showing no evidence of protection.
  • There was no observed correlation between pharmacokinetics (maraviroc concentrations) and pharmacodynamics (antiviral activity), and there was substantial pharmacodynamic variability.
  • By day 15 there was no association between p24 levels and maraviroc concentrations in blood plasma, vaginal tissue, or rectal tissue.

Based on these findings the researchers concluded that high maraviroc concentrations were detectable in vaginal and rectal tissue at 4 hours after a single maraviroc dose. However, "No ex vivo protection or PK/PD relationship was observed in vaginal or rectal tissue" after a single 300 mg maraviroc dose.

"Although no protective effect of single dose maraviroc [was] seen, this does not preclude an effect with multiple maraviroc dosing," they added, noting that further studies are underway.

Following the presentation attendees discussed the possible meaning of these findings. Session moderator Marta Boffito from Chelsea and Westminster Hospital stressed that "methodologies need to be optimized and standardized...we're starting to see interesting results but they're not always in agreement." She suggested that this type of research should include a tenofovir comparison, since it has a known protective effect.

Ian McGowan from the University of Pittsburgh said that the "devil is always in the details" with regard to the timing of testing, since there is evidence of very rapid dissociation of maraviroc from tissue.

Craig Hendrix from Johns Hopkins cautioned that when the same type of study was done using tenofovir -- looking at p24 levels in tissue samples after a single oral -- it also showed no protective effect. "It's important to understand the relationship between in vitro, ex vivo, and in vivo testing," he said. "If we had made any decisions for tenofovir based on the same testing with oral dosing, we never would have studied it -- but it works when you take it."

3/2/15

Reference

J Fox, C Herrera, JM Tiraboschi, et al. A Phase IV PrEP Study Reveals Limited Ex Vivo Potency of Oral Maraviroc Against HIV-1. 2015 Conference on Retroviruses and Opportunistic Infections. Seattle, February 23-24, 2015. Abstract 86LB.